Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1
نویسندگان
چکیده
منابع مشابه
XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. Here, we describe our characterization of XatA, the X. fastidiosa autotransporte...
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The Restriction-modification system AhdI contains two convergent transcription units, one with genes encoding methyltransferase subunits M and S and another with genes encoding the controller (C) protein and the restriction endonuclease (R). We show that AhdI transcription is controlled by two independent regulatory loops that are well-optimized to ensure successful establishment in a naïve bac...
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The purpose of this study is to elucidate the contributions of host cell wall-degrading enzymes (CWDEs) produced by Xylella fastidiosa (Xf) to systemic colonization of grapevine, as well as the role of the Type II Secretion System (T2SS) in delivering these CWDEs into the xylem. Of the CWDEs predicted to be secreted by the T2SS, this project will focus on the endoglucanases (EGases) produced by...
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Xylella fastidiosa is a xylem-limited, gram-negative bacterium that causes Pierce's disease of grapevine. Here, we describe the construction of four vectors that facilitate the insertion of genes into a neutral site (NS1) in the X. fastidiosa chromosome. These vectors carry a colE1-like (pMB1) replicon and DNA sequences from NS1 flanking a multiple-cloning site and a resistance marker for one o...
متن کاملMapping analysis of the Xylella fastidiosa genome.
A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2010
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03034-09